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Reconstitute Rho1D4 peptide in 0.53?mL ddH20 to obtain a 10 mM stock solution.
Dilute stock solution in elution buffer directly before use as follows:
Final Rho1D4 peptide concentration in elution buffer - Dilution of stock solution - Final volume of elution buffer
200 μM - 1:50 - 26.45?mL
400 μM - 1:25 - 13.26?mL
500 μM - 1:20 - 10.58?mL
800 μM - 1:12.5 - 6.62?mL
1000 μM - 1:10 - 5.29?mL
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