產(chǎn)品詳情

  • 產(chǎn)品名稱：Mouseanti-HumanIgGAntibody

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  • 產(chǎn)品廠商：NSJBio
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Mouseanti-HumanIgGAntibody
詳情介紹：
Immunogen Purified human IgG was used as the immunogen for this IgG antibody.
Clone IG217-IG266
Isotype IgG1, IgG2a
Characteristics This mAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of gamma heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
Purification Protein G affinity chromatography
Background This mAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of gamma heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
Research Area Immunology, Secondary Antibodies
Application Notes The optimal dilution of the IgG antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10?mM citrate buffer,?pH 6.0, for 10-20?min followed by cooling at RT for 20?minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30?min.\. Prediluted format : incubate for 30?min at RT (2)
Restrictions For Research Use only
Format Liquid
Buffer Prediluted in 1X PBS with 0.1?mg/mL BSA (US sourced) and 0.05?% sodium azide, *For IHC use only*
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the IgG antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).