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  • 產(chǎn)品名稱：Mouseanti-HumanIgMAntibody

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簡單介紹：
Mouseanti-HumanIgMAntibody
詳情介紹：
Immunogen Heavy chain of human IgM was used as the immunogen for the Anti-IgM antibody.
Clone SPM188
Isotype IgG1, kappa
Purification Protein G affinity chromatography
Background Recognizes a protein of 75?kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Research Area Secondary Antibodies
Application Notes Optimal dilution of the Anti-IgM antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10?mM Citrate buffer,?pH 6.0, for 10-20?min followed by cooling at RT for 20?min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30?min.\. Flow Cytometry: 0.5-1?μg/million cells in 0.1ml,Immunofluorescence: 0.5-1?μg/mL,Immunohistochemistry (FFPE): 0.5-1?μg/mL for 30?min at RT (1),Prediluted format : incubate for 30?min at RT (2)
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 0.2?mg/mL in 1X PBS with 0.1?mg/mL BSA (US sourced) and 0.05?% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Anti-IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Supplier Images
Immunohistochemistry (IHC) image for Mouse anti-Human IgM antibody (ABIN3025946) Formalin-fixed, paraffin-embedded human tonsil stained with anti-IgM antibody (SPM188)