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  • 產(chǎn)品名稱：Mouseanti-HumanIgEAntibody(Biotin)

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簡單介紹：
Mouseanti-HumanIgEAntibody(Biotin)
詳情介紹：
Immunogen purified human myeloma IgE
Clone BSW17-NBS01
Isotype IgG1 kappa
Specificity Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
Sensitivity Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)
Characteristics IGHE, chromosome 14q32.33
Purification Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
Purity > 85 %, tested by analytical Gel Permeation Chromatography
Background The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
Research Area Immunology, Secondary Antibodies
Application Notes When used for functional cellular assays at dilutions < 1:100, the Na-azide free formulation (Cat. No. 0910-1-010 / 050-B) is recommended
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, pH=7.2, 0.1 % NaN3
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Aliquot to Avoid repeated freezing and thawing.
Storage 4 °C
Supplier Images
image for Mouse anti-Human IgE antibody (Biotin) (ABIN457501) product purity: gel permeation chromatography (Superose 12/HR)
ELISA image for Mouse anti-Human IgE antibody (Biotin) (ABIN457501) ELISA binding to human IgE(SUS-11), Detection: Streptavidin-HRP