產(chǎn)品詳情

  • 產(chǎn)品名稱：MBPSelector

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  • 產(chǎn)品廠商：Nano-Tag
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簡單介紹：
MBPSelector
詳情介紹：
Sample Type Cell Extracts
Specificity The Antibody with Clone 1G5 recognizes E.coli maltose-binding protein (MBP)
Characteristics MBP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4?% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. MBP Selector thus features high affinity and superior capacity for MBP fusion proteins while showing negligible non-specific background. MBP Selector is compatible not only with physiological buffers but also with high stringency buffers. MBP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Components 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
Material not included wash buffers, columns, tubes
ProductDetails: Bead Ligand Antibody
ProductDetails: Bead Matrix Agarose beads
ProductDetails: Bead Size 90 μm
Alternative Name maltose binding protein, MBP
Background Maltose-binding protein (MBP) is encoded by the malE gene from the gram-negative bacterium Escherichia coli. When expressed as a fusion protein it boosts the expression of the fusion partner and is therefore a common entity in bacterial expression vectors
Application Notes 200?μL slurry for up to 10 reactions
Comment

Capacity: > 2.5 μg MBP per μl of packed beads (i.e. 2 μl of slurry)
Restrictions For Research Use only
Buffer 50?% slurry in 20?% ethanol / PBS 0.09?% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C