Immunofluorescence staining of HER2 protein on SK-BR-3 cells. Cells were cultured on cover slips. After fixation and permeabilization, cells were incubated with rabbit anti-HER2 mAb (CST), followed by detection with IRDye? 650 Goat anti-Rabbit (LI-COR P/N 926-65020). Nuclei were stained with DAPI. Image acquired with Olympus IX81 microscope.