產(chǎn)品詳情

  • 產(chǎn)品名稱：Caspase-3/CPP32Substrate-pNA

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  • 產(chǎn)品廠商：KamiyaBiomedical
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Caspase-3/CPP32Substrate-pNA
詳情介紹：
Purpose Soluble in DMSO and aqueous buffers. We recommend preparing a stock solution in high purity DMSO (>99.9%), and diluting into aqueous buffer shortly prior to use.
1. Lyse cells in 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT, at a density of 2 x 10^6 /mL.
2. Assay 0.01 mL cell lysate in a final volume of 0.1 mL. Assay buffer is cell lysis buffer containing 0.2 mM substrate.
3. Incubate at 37°C for 0-3 hr. Take periodic readings of absorbance at 405 nm.
Sequence Acetyl-Asp-Glu-Val-Asp-pNA, Ac-DEVD-pNA
Specificity A substrate for caspase-3 and related cysteine proteases. Also cleaved by caspase-6, caspase-7, caspase-8 and caspase-10.
Purity > 97 % by HPLC
Background Chromogenic paranitroanilide-peptide substrate for caspase-3 (Km = 9.7 μM). Ac-DEVD-pNA is based on amino acids 213-216 in poly (ADP-ribose) polymerase (PARP), an endogenous substrate for caspase-3. This substrate is also cleaved by caspases-6, -7, -8, and -10. Release of free pNA is monitored by absorbance at 405 nm ( = 9,160 M - 1 cm -1 ). Caspase-3/CPP32 is a member of the cysteine proteases family involved in apoptosis induction. All apoptotic pathways studied to date involve proteolytic activation of Caspase-3/CPP32 as a central event in the progression of cell death. Although the death-inducing consequences of Caspase-3/CPP32 activation have not been conclusively established, several crucial substrates for the protease have been identified in vitro, including DNA-dependent protein kinase, Poly (ADP-ribose) Polymerase (PARP), Replication factor C, and Gelsolin. These substrates are involved in the later stages of apoptosis, strongly suggesting that Caspase-3/CPP32 has a key role in promoting the final processes leading to cell death.
Molecular Weight 638 Da
Application Notes Assay of caspase activity in cell extracts.
Comment

Formula: C26H34N6O13
Restrictions For Research Use only
Format Lyophilized
Storage RT