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Characteristics
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HiLyte Fluor? 750-IgG conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised IgG function. Spectrally similar to Cy7 dye, HiLyte Fluor? 750 is the longest-wavelength HiLyte Fluor? dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte Fluor? 647, HiLyte Fluor? 680 or allophycocyanin (APC), facilitating multicolor analysis. With a peak
Excitation at ~754 nm, conjugates of HiLyte Fluor? 750 dyes are well excited by a xenon-arc lamp or dye-pumped lasers operating in the 720-750 nm range. The fluorescence signal of HiLyte Fluor? 750-conjugate can be observed at 778 nm with the
Excitation at 754 nm.
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