產(chǎn)品詳情

  • 產(chǎn)品名稱：CCL24 ELISA Kit(Chemokine(C-CMotif)Ligand24)

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
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CCL24 ELISA Kit(Chemokine(C-CMotif)Ligand24)
詳情介紹：
Purpose This immunoassay kit allows for the specific measurement of mouse Eotaxin-2 (CCL24) concentrations in cell culture supernates, serum, plasma , BALF and tissue homogenates.
Sample Type Cell Culture Supernatant, Serum, Plasma, Body Fluids, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse CCL24. The factors listed below were assayed for cross-reactivity.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Characteristics Mus musculus,Mouse,C-C motif chemokine 24,Eosinophil chemotactic protein 2,Eotaxin-2,Small-inducible cytokine A24,Ccl24,Scya24
Alternative Name Ccl24 (CCL24 ELISA Kit Abstract)
Background Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and Il-4. LPS and IL-4 also differentially regulate the expression of eotaxin-2 in monocytes and macrophages. Mouse eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human eotaxin-2. Functionally, eotaxin-2 is most closely related to eotaxin/CCL11 and eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.
Pathways Regulation of Actin Filament Polymerization
Sample Volume 100 μL
Plate Pre-coated
Protocol This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CCL24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL24 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CCL24 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.