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  • 產(chǎn)品名稱：COL1A1 ELISA Kit(Collagen,TypeI,alpha1)

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
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COL1A1 ELISA Kit(Collagen,TypeI,alpha1)
詳情介紹：
Purpose This immunoassay kit allows for the specific measurement of mouse Collagen Type Ⅰ concentrations in cell culture supernates, serum, and plasma.
Sample Type Cell Culture Supernatant, Serum, Plasma
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse Collagen Type Ⅰ.
Characteristics Mus musculus,Mouse,Collagen alpha-1(I) chain,Alpha-1 type I collagen,Col1a1,Cola1
Alternative Name Col1a1 (COL1A1 ELISA Kit Abstract)
Background Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600? in length and 15? in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Type 1 collagen is the most abundant protein in the connective tissues, especially in tendon, skin and bone.
Pathways Sensory Perception of Sound, Autophagy, Growth Factor Binding
Sample Volume 100 μL
Plate Pre-coated
Protocol This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Collagen Type Ⅰ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Collagen Type Ⅰ present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Collagen Type Ⅰ is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Collagen Type Ⅰ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.