產(chǎn)品詳情

  • 產(chǎn)品名稱：F2RL1 ELISA Kit(CoagulationFactorII(thrombin)Receptor-Like1)

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
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F2RL1 ELISA Kit(CoagulationFactorII(thrombin)Receptor-Like1)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of Human TPS, concentrations in serum, tissue homogenates and other biological fluids.
Sample Type Serum, Tissue Homogenate, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural human TPS.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 0.036 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Homo sapiens,Human,Proteinase-activated receptor 2,PAR-2,Coagulation factor II receptor-like 1,G-protein coupled receptor 11,Thrombin receptor-like 1,F2RL1,GPR11,PAR2
Alternative Name F2RL1 (F2RL1 ELISA Kit Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TPS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TPS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.