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                            產(chǎn)品詳情
                        
    
                        
    
                            
    
                                
                            
    
                            
    
                                
                                
                            
    
                            

                            
    
    
    
    
    
        
    
    
    
            
    

                
    
                    
                    
      
                          
    • 產(chǎn)品名稱：IL-10 ELISA Kit(Interleukin10)
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    • 產(chǎn)品型號：
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    • 產(chǎn)品廠商：國內供應3
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    • 產(chǎn)品文檔：
                          
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            簡單介紹：
        
    
        
    
            IL-10 ELISA Kit(Interleukin10)
        
    
    
    

    
    
        
    
            詳情介紹：
        
    
        
    
            
    
                
    
                    
                        
                        
                    
                    
                                                                                                                                                        
    
                                                                                    
                                                Purpose
                                            
                                        
                                                                                    
                                            
                                                This immunoassay kit allows for the in vitro quantitative determination of mouse Interleukin 10,IL-10 concentrations in cell culture supernates, serum, plasma and other biological fluids.
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
    
                                                                                    
                                                Sample Type
                                            
                                        
                                                                                    
                                            
                                                Cell Culture Supernatant, Serum, Plasma, Biological Fluids
                                                                                            
                                                                            
    
                                                                                                                                                                
    
                                                                                    
                                                Analytical Method
                                            
                                        
                                                                                    
                                            
                                                Quantitative
                                                                                            
                                                                            
    
                                                                                                                                                                
    
                                                                                    
                                                Detection Method
                                            
                                        
                                                                                    
                                            
                                                Colorimetric
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
    
                                                                                    
                                                Specificity
                                            
                                        
                                                                                    
                                            
                                                This assay recognizes recombinant and natural mouse IL-10.
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                                                                                                                        
    
                                                                                    
                                                Cross-Reactivity (Details)
                                            
                                        
                                                                                    
                                            
                                                No significant cross-reactivity or interference was observed.
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                            
    
                                                                                    
                                                Sensitivity
                                            
                                        
                                                                                    
                                            
                                                < 7.8 pg/mL
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
                                                                                                		
                                                                            
    
                                                                                                                                                                
    
                                                                                    
                                                Characteristics
                                            
                                        
                                                                                    
                                            
                                                Mus musculus,Mouse,Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,Il10,Il-10
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
                        
                
    
            
    
                                                                        
    
                
    
                    
                        
                        
                    
                    
                                                                                                                                                        
    
                                                                                    
                                                Alternative Name
                                            
                                        
                                                                                    
                                            
                                                Il10 (IL10  ELISA Kit Abstract)
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                                                                                                                        
    
                                                                                    
                                                Background
                                            
                                        
                                                                                    
                                            
                                                Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 cytokine family. This family currently comprises IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. All known IL-10 family members are secreted -helical proteins. IL-10 is a secreted, possibly glycosylated, polypeptide with a molecular weight of 18 kDa. Based on human studies, IL-10 is likely to circulate as a nondisulfide-linked homodimer. IL-10 is synthesized as a 181 amino acid (aa) precursor with a 19 aa signal sequence and a 162 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which likely form two intra-chain disulfide bridges. Mature rat IL-10 shows 76%, 73%, 72%, 80%, 86%, 85% and 76% aa identity to guinea pig, mouse, human, feline and equine IL-10, respectively. Upon activation, mammalian cells known to secrete IL-10 include NK cells, cytotoxic CD8+ T cells secreting Th2-like cytokines, CD4+CD45RA- (memory) Th1 and Th2 cells, macrophages, monocytes, CD5+ and CD5- B cells, dendritic cells, hepatic stellate (Ito) cells, keratinocytes, melanoma cells, mast cells, placental cytotrophoblasts, and fetal erythroblasts.
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
    
                                                                                    
                                                Pathways
                                            
                                        
                                                                                    
                                            
                                                Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
                                                                                            
                                                                            
    
                                                                                    
                        
                
    
            
    
                                                            
    
                
    
                    
                        
                        
                    
                    
                                                                                                                                                                                                                                                                                                                                                
    
                                                                                    
                                                Sample Volume
                                            
                                        
                                                                                    
                                            
                                                100 μL
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                            
    
                                                                                    
                                                Plate
                                            
                                        
                                                                                    
                                            
                                                Pre-coated
                                                                                            
                                                                            
    
                                                                                                                                                                
    
                                                                                    
                                                Protocol
                                            
                                        
                                                                                    
                                            
                                                The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
                                                                                            
                                                                            
    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
    
                                                                                    
                                                Restrictions
                                            
                                        
                                                                                    
                                            
                                                For Research Use only
                                                                                            
                                                                            
    
                                                                                    
                        
                
    
            
    
                                                                                                
    
                
    
                    
                        
                        
                    
                    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
    
                                                                                    
                                                Storage
                                            
                                        
                                                                                    
                                            
                                                4 °C/-20 °C
                                                                                            
                                                                            
    
                                                                                                                                                                
    
                                                                                    
                                                Storage Comment
                                            
                                        
                                                                                    
                                            
                                                The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.