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  • 產(chǎn)品名稱：IL-4 ELISA Kit(Interleukin4)

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)3
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IL-4 ELISA Kit(Interleukin4)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouse IL-4 concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural mouse IL-4.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity < 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Mus musculus,Mouse,Interleukin-4,IL-4,B-cell IgG differentiation factor,B-cell growth factor 1,B-cell stimulatory factor 1,BSF-1,IGG1 induction factor,Lymphocyte stimulatory factor 1,Il4,Il-4
Alternative Name Il4 (IL4 ELISA Kit Abstract)
Background Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes,mast cells and basophils . IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivoand in vitro. The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit (IL-4 R) and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-2, IL-7, IL-9 and IL-15. A second type of functional IL-4 receptor complex, consisting of the IL-4 R and the recently cloned IL-13 R, has also been proposed. Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13.
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of IL-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.