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Characteristics
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EDTA Buffer, after dilution, is used on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides for target retrieval prior to immunohistochemistry (IHC) procedures. When performed using this reagent and elevated temperature, target retrieval can disrupt the covalent bonds formed by formalin in tissue. Removal of these bonds can allow renaturation of protein molecules and increased antibody accessibility, which in turn can result in increased antibody binding (and thus increased staining intensity) and improved signal to noise ratios. This type of result is observed with many primary antibodies.
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