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Characteristics
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Features: Jacalin-Sepharose beads are prepared by covalently coupling highly purified Jacalin to 6 % cross-linked Sepharose beads. The coupling technique is optimized to give a higher binding capacity for IgA & minimal leaching of Jacalin. The binding capacity of Jacalin-Sepharose is ≥ 3 mg human IgA per mL of wet beads
Applications: Purification of human IgA from serum or colostrum samples or removal of contaminating IgA from IgG samples. Jacalin, an alpha-D-galactose binding lectin extracted from Jackfruit seeds, binds with high specificity to human IgA1 and other o-glycoproteins such as mucin or glycopeptides. high-quality Jacalin-Sepharose? exhibits specific, high-yield purification of human IgA from serum or colostrum and thus can also be used for removing contaminating IgA from IgG. Jacalin can also be used to separate IgA1 subclass from IgA2. Precautions should be taken while purifying antibodies produced in the presence of bovine serum as Jacalin is capable of binding to bovine glycoproteins, e.g. feutin that may interfere with the purification.
Synonyms: Jacalin-Sepharose
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