產(chǎn)品詳情

  • 產(chǎn)品名稱：ProteinMolecularWeightMarker

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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ProteinMolecularWeightMarker
詳情介紹：
Purpose This protein molecular weight marker is a cocktail containing seven purified proteins that range in size from 14 to 120 kDa and thus span a molecular weight range common to most proteins and protein subunits.
Detection Method Colorimetric
Characteristics The protein mixture has been formulated to yield well-defined bands after SDS-polyacrylamide gel electrophoresis and staining with Coomassie blue or other common staining techniques. The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference. The product is supplied in 1X loading buffer and is ready-to-use.
Sterility Sterile filtered
Application Notes This product is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10μl is appropriate for most gel formats. These are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). 62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue
Comment

in 1x Loading Buffer
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
image for Protein Molecular Weight Marker (ABIN925595) Protein Molecular Weight Marker
image for Protein Molecular Weight Marker (ABIN925595) Protein Molecular Weight Marker (Image 2)