產(chǎn)品詳情
簡單介紹:
HumanIgGIsotypeControl
詳情介紹:
Clone | 4D2D9G8 |
Isotype | IgG |
Fragment | Fab fragment |
Characteristics |
Human IgG was isolated from human sera and purified by chromatography. Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor. |
Purification | Affinity Chromatography on Protein G |
Research Area | Immunology, Secondary Antibodies |
Application Notes | ELISA: 1: 5000, WB: 1: 500-1: 2000 |
Restrictions | For Research Use only |
Buffer | 0.1 mg IgG in PBS pH 7.4 with 0.03 % sodium azide. |
Preservative | Sodium azide |
Precaution of Use | Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | -20 °C |
Storage Comment | Store at 4 °C for short term use only. Store at -20 °C for storage over 1 month. |
Expiry Date | 6 months |
Supplier Images |
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