產(chǎn)品詳情
簡單介紹:
Mouseanti-Rat(Rattus)IgG1(HeavyChain)Antibody
詳情介紹:
Immunogen | Rat IR27 IgG1 myeloma. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
Clone | MARG1-2 |
Isotype | IgG1 |
Purification | Affinity Chromatography |
Background | Synonyms: Rat Immunoglobulin G1 |
Research Area | Secondary Antibodies |
Application Notes |
ELISA: 3?μg/mL - 3?μg/mL, This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgG1 (SA013). Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Restrictions | For Research Use only |
Concentration | 1.0 mg/mL |
Buffer | PBS,?pH 7.4, 0.09?% Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment | Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer. |
Background publications |
Sato, Balla, Otterbein, Smith, Brouard, Lin, Csizmadia, Sevigny, Robson, Vercellotti, Choi, Bach, Soares: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001 (PubMed).
Pelegrí, Castell, Serra, Rabanal, Rodríguez-Palmero, Castellote, Franch: "Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells." in: Clinical and experimental immunology, Vol. 125, Issue 3, pp. 470-7, 2001 (PubMed). |