產(chǎn)品詳情
簡(jiǎn)單介紹:
Mouseanti-Rat(Rattus)IgA(HeavyChain),(Chainalpha)Antibody
詳情介紹:
Immunogen | Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
Clone | MARA-1 |
Isotype | IgG1 |
Purification | Affinity Chromatography |
Background | Synonyms: Rat Immunoglobulin A |
Research Area | Immunology, Secondary Antibodies |
Application Notes |
ELISA: 5?μg/mL, This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgA (SA013). Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Restrictions | For Research Use only |
Concentration | 1 mg/mL |
Buffer | PBS,?pH 7.4 containing 0.09?% Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment | Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer. |
Background publications |
Hahn, Thiessen, Pabst, Buettner, Bode: "Mesenteric lymph nodes are not required for an intestinal immunoglobulin A response to oral cholera toxin." in: Immunology, Vol. 129, Issue 3, pp. 427-36, 2010 (PubMed).
Budec, Markovi?, Djiki?, Mitrovi?, Drndarevi?, Koko, Todorovi?: "Blockade of nitric oxide synthesis modulates rat immunoglobulin A." in: Neuroimmunomodulation, Vol. 16, Issue 3, pp. 155-61, 2009 (PubMed). Budec, Koko, Todorovi?, Markovi?, Posti?, Drndarevi?, Spasi?, Mitrovi?: "Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat." in: International immunopharmacology, Vol. 7, Issue 6, pp. 858-63, 2007 (PubMed). Bjersing, Telemo, Dahlgren, Hanson: "Loss of ileal IgA+ plasma cells and of CD4+ lymphocytes in ileal Peyer's patches of vitamin A deficient rats." in: Clinical and experimental immunology, Vol. 130, Issue 3, pp. 404-8, 2002 (PubMed). Bazin, Beckers, Querinjean: "Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c." in: European journal of immunology, Vol. 4, Issue 1, pp. 44-8, 1974 (PubMed). |