產(chǎn)品詳情
簡(jiǎn)單介紹:
Mouseanti-Rat(Rattus)IgA(HeavyChain)Antibody(FITC)
詳情介紹:
Immunogen | Purified IR1060 IgA rat myeloma protein |
Clone | MARA-1 |
Isotype | IgG1 |
Purification | Affinity chromatography |
Background | Synonyms: Rat Immunoglobulin A |
Research Area | Immunology, Secondary Antibodies |
Application Notes |
Flow cytometry (5?μg/mL): use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Comment |
Excitation/Emission wavelength: 494 nm/514 nm |
Restrictions | For Research Use only |
Concentration | 1.0 mg/mL |
Buffer | PBS containing 50?% glycerol and 0.09?% sodium azide |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice |
Product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment | Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer. |
Background publications |
Bazin, Cormont, De Clercq: "Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum." in: Journal of immunological methods, Vol. 71, Issue 1, pp. 9-16, 1984 (PubMed).
Bazin, Beckers, Urbain-Vansanten, Pauwels, Bruyns, Tilkin, Platteau, Urbain: "Transplantable IgD immunoglobulin-secreting tumors in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 121, Issue 5, pp. 2077-82, 1979 (PubMed). Bazin, Beckers, Querinjean: "Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c." in: European journal of immunology, Vol. 4, Issue 1, pp. 44-8, 1974 (PubMed). GRABAR, WILLIAMS: "[Method permitting the combined study of the electrophoretic and the immunochemical properties of protein mixtures; application to blood serum]." in: Biochimica et biophysica acta, Vol. 10, Issue 1, pp. 193-4, 1953 (PubMed). |