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  • 產(chǎn)品名稱：Mouseanti-HumanIgMAntibody

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  • 產(chǎn)品廠商：NSJBio
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Mouseanti-HumanIgMAntibody
詳情介紹：
Immunogen Human Mu heavy chain was used as the immunogen for this IgM antibody.
Clone MuHC2
Isotype IgG1, kappa
Purification Protein G
Background IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen s (due to the large size of most antigens, not all s can be filled simultaneously. IgM antibodies account for approximately 5?% -10?% of all the antibody in the body.
Gene ID 3507
Research Area Secondary Antibodies
Application Notes Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling sections in 10?mM citrate buffer,?pH 6.0, for 10-20?min followed by cooling at RT for 20?minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30?min.\. Immunofluorescence: 0.5-1?μg/mL,Western blot: 0.5-1?μg/mL,IHC (FFPE): 0.5-1?μg/mL for 30?minutes at RT (1),Prediluted format : incubate for 30?min at RT (2)
Restrictions For Research Use only
Buffer 1?mg/mL in 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store the IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).