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  • 產(chǎn)品名稱：Mouseanti-HumanIgGAntibody

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  • 產(chǎn)品廠商：NSJBio
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簡單介紹：
Mouseanti-HumanIgGAntibody
詳情介紹：
Immunogen Purified human IgG was used as the immunogen for this IgG antibody.
Clone IG217-IG266
Isotype IgG1, IgG2a
Characteristics This mAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of gamma heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
Purification Protein G affinity chromatography
Background This mAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of gamma heavy chain (IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.
Research Area Immunology, Secondary Antibodies
Application Notes The optimal dilution of the IgG antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10?mM citrate buffer,?pH 6.0, for 10-20?min followed by cooling at RT for 20?minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30?min.\. Flow Cytometry (1?μg/million cells in 0.1ml),Immunofluorescence: 0.5-1?μg/mL,Immunohistochemistry (FFPE): 0.5-1?μg/mL for 30?minutes at RT (1),Prediluted format : incubate for 30?min at RT (2)
Restrictions For Research Use only
Buffer 1?mg/mL in 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store the IgG antibody c°Cktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).