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  • 產(chǎn)品名稱：Mouseanti-HumankappaLightChainAntibody

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Mouseanti-HumankappaLightChainAntibody
詳情介紹：
Immunogen Purified human Ig kappa chain was used as the immunogen for this Kappa Light Chain antibody.
Clone HP6053
Isotype IgG1, kappa
Characteristics This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Purification Protein G affinity chromatography
Background This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Research Area Secondary Antibodies
Application Notes The optimal dilution of the Kappa Light Chain antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10?mM Citrate Buffer,?pH 6.0, for 10-20?min followed by cooling at RT for 20?minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30?min.\. Flow Cytometry (0.5-2.0?μg/million cells in 0.1ml),Western blot: 0.5-1?μg/mL,Immunohistochemistry (FFPE): 0.5-1?μg/mL for 30?min at RT (1),Prediluted format : incubate for 30?min at RT (2)
Restrictions For Research Use only
Buffer 1?mg/mL in 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store the Kappa Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).