產(chǎn)品詳情
簡(jiǎn)單介紹:
Rabbitanti-HumanImmunoglobulinG4(IgG4)Antibody
詳情介紹:
Immunogen | Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody. |
Clone | RM120 |
Isotype | IgG |
Characteristics | This recombinant Human IgG4 antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
Purification | Protein A purified from animal origin-free supernatant |
Alternative Name | IgG4 |
Gene ID | 3503 |
UniProt | P01861 |
Research Area | Secondary Antibodies |
Application Notes |
The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. A?pH 6 Citrate buffer or?pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 50?ng/well - 200?ng/well (Capture), 0.05-0.2?μg/mL (Detection),Western Blot: 0.5-2?μg/mL,Immunocytochemistry: 1-10?μg/mL,Immunohistochemistry: 1-10?μg/mL (1) |
Restrictions | For Research Use only |
Buffer | 1?mg/mL in PBS with 50?% glycerol, 1?% BSA and 0.09?% sodium azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | -20 °C |
Storage Comment | Store the recombinant Human IgG4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol). |