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Sample Type
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Cell Extracts
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Specificity
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The Antibody with Clone 1H1 recognizes GFP, mEGFP, superfolder GFP, Emerald GFP and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
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Characteristics
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GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4?% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background. GFP Selector immobilizes a wide range of GFP derivatives. For details see our Product Specificity Chart below. GFP Selector is compatible not only with physiological buffers but also with high stringency buffers. GFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
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Components
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4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
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Material not included
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wash buffers, columns, tubes
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ProductDetails: Bead Ligand
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Antibody
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ProductDetails: Bead Matrix
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Agarose beads
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ProductDetails: Bead Size
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90 μm
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