A recombinant variant of the human TSH receptor (70.8 kDa) was subjected to Western blotting. Upon incubation with the primary antibody (mixture of human Graves disease autoimmune sera), the membrane was washed and incubated with Goat anti-Human-HRP (1:1,000). Colorimetric detection.
Determination of human autoantibodies with Goat anti-Human-HRP. A recombinant variant of the human TSH receptor was immobilized onto an ELISA-plate and incubated with a mixture of human Graves disease autoimmune sera. Bound autoantibodies were determined with Goat anti-Human-HRP at the indicated dilution factors. Visualization was performed with a commercially available TMB ELISA substrate