Immunofluorescence staining of tubulin protein in HeLa cells. Cells were cultured on cover slips. After fixation and permeabilization, cells were incubated with rabbit anti-tubulin mAb (CST), followed by VRDye? 490 Goat anti-Rabbit IgG. Nuclei were stained with DAPI. Image acquired with Olympus IX81 microscope.