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  • 產(chǎn)品名稱：Donkeyanti-ChickenIgG(Heavy&LightChain)Antibody(IRDye680LT)

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  • 產(chǎn)品廠商：LI-COR
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Donkeyanti-ChickenIgG(Heavy&LightChain)Antibody(IRDye680LT)
詳情介紹：
Immunogen Chicken IgY, whole molecule. IgY is the original destination for the IgG-like protein found in both chicken serum and egg yolk.
Isotype IgG
Fragment Whole molecule
Specificity Chicken IgY
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY.
Cross-Reactivity (Details) This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, goat, guinea pig, Syrian hamster, horse, mouse, human, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species.
Characteristics It also reacts with the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Purification Immunoaffinity chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Western blot: 1:20,000 Recommended
Optimum dilutions will vary and should be determined empirically.

NOTE: When using PVDF membranes for Western blotting, SDS (final concentration of 0.1 – 0.2%) and Tween? 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

  • IRDye 680LT secondary antibodies are suitable for immunofluorescent microscopy and other fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
  • IRDye 680LT is not intended for In-Cell WesternTM assays or in vivo applications.
  • These antibodies are not recommended when getting up and running on system. Once established near-infrared protocols are optimized with IRDye 680RD, IRDye 680LT can be used to optimize signals in the 700 channel. Dilution range 1:20,000 – 1:40,000.
    Note: optimization may be required with IRDye 680LT. Please see pack insert for specific detergents/protocol modifications needed.
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    Comment

    • IRDye 680LT secondary antibodies have been proven to provide the brightest signal for Western blot detection in the 700 nm channel and are comparable to Alexa Fluor? 680 secondary antibodies.
    • Choose IRDye 680LT secondary antibodies to get high signal and for specific uses of detection in the 700nm channel.
    • We do not recommend to start with IRDye 680LT secondaries but rather to start with one of the IRDye 680RD secondaries.

    Restrictions For Research Use only
    Format Lyophilized
    Reconstitution Reconstitute with sterile, distilled water
    Concentration 1.0 mg/mL
    Buffer PBS,?pH 7.4
    Preservative Sodium azide
    Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice Protect from light.
    Storage 4 °C
    Product cited in: Matsuo, Coon, Klein: "RGS2 is a feedback inhibitor of melatonin production in the pineal gland." in: FEBS letters, Vol. 587, Issue 9, pp. 1392-8, 2013 (PubMed).