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  • 產(chǎn)品名稱：S100A12 ELISA Kit(S100CalciumBindingProteinA12)

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  • 產(chǎn)品廠商：國內(nèi)供應(yīng)2
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S100A12 ELISA Kit(S100CalciumBindingProteinA12)
詳情介紹：
Purpose This ELISA Kit applies to the in vitro quantitative determination of Guinea pig S100A12 concentrations in serum, plasma and other biological fluids.
Sample Type Plasma, Serum, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This kit recognizes natural and recombinant Guinea pig S100A12. No significant cross-reactivity or interference between Guinea pig S100A12 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Guinea pig S100A12 and all the analogues.
Sensitivity 0.094 ng/mL
Pathways Toll-Like Receptors Cascades
Sample Volume 100 μL
Plate Pre-coated
Protocol This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to S100A12. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100A12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100A12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of S100A12. You can calculate the concentration of S100A12 in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
Restrictions For Research Use only
Storage Comment All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20°C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur. 6th Edition, revised in June, 2015 www.elabscience.com 3