產(chǎn)品詳情

  • 產(chǎn)品名稱：MouseImmunoglobulinG2a(IgG2a)isotypecontrol(FITC)

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  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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簡單介紹：
MouseImmunoglobulinG2a(IgG2a)isotypecontrol(FITC)
詳情介紹：
Clone ICIGG2A
Isotype IgG2a
Characteristics This mouse monoclonal IgG2a was produced against a synthetic hapten, which is normally not present in humans or animals. Therefore, this mouse IgG2a does not react with any antigen of human or animal cells.
Alternative Name IgG2a
Research Area Secondary Antibodies
Application Notes It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 μL/10^6 cells.
Comment

Fluorescein isothiocyanate (Molecular Probes).
Excitation/Emission wavelength: 494 nm/514 nm
Sample Preparation
  1. Transfer 100 μL of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 μL of Isotype Control IgG2a and mix gently with a vortex mixer. The 20 μL is a guideline only, the correct volume depends on the quantity of direct monoclonal used. 3. Incubate in the dark at room temperature at 4 °C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 4. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 5. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 μL of fluid. 6. Add 2 mL 0.01 mol/l PBS (It betters that it containing 2 % bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 μL of fluid. 8. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1 % paraformaldehyde (fixative) if samples are not analysed the same day. 9. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
Restrictions For Research Use only
Preservative Sodium azide
Precaution of Use 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
Handling Advice Product is photosensitive and should be protected from light.
Storage 4 °C