產(chǎn)品詳情
簡(jiǎn)單介紹:
RatImmunoglobulinG2a(IgG2a)IsotypeControl
詳情介紹:
Clone | RG2aL |
Isotype | IgG2a |
Characteristics |
Sex: Female |
Purification | RAT IgG2a Lambda (λ) isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed. |
Sterility | Sterile filtered |
Alternative Name | IgG2a |
Research Area | Secondary Antibodies |
Application Notes |
Flow Cytometry Dilution: 1:1000-1:5000 Application Note: RAT IgG2a Lambda (λ) isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user. Western Blot Dilution: User Optimized FLISA Dilution: User Optimized ELISA Dilution: User Optimized IF Microscopy Dilution: User Optimized |
Restrictions | For Research Use only |
Format | Liquid |
Concentration | 1.1 mg/mL |
Buffer |
Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride,?pH 7.2 Stabilizer: None |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C,-20 °C |
Storage Comment | Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Expiry Date | 12 months |