產(chǎn)品詳情

  • 產(chǎn)品名稱：TrueBlot?Anti-MouseIgIPBeads?

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：Rockland
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簡單介紹：
TrueBlot?Anti-MouseIgIPBeads?
詳情介紹：
Brand TrueBlot?
Characteristics TrueBlot? anti-Mouse Ig IP Beads are a suspension of activated agarose beads coupled with goat anti-mouse IgG. It is suitable for precipitation of mouse IgGs used as the primary antibodies in immunoprecipitation assays. The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Conjugation Name: Agarose beads for TrueBlot?
Components TrueBlot? Anti-Mouse Ig IP Beads
Research Area Immunology, Secondary Antibodies
Application Notes Immunoprecipitation Dilution: TrueBlot? anti-Mouse Ig IP Beads (binds 0.4 mg Ig/mL beads) have been reported for use in IP
Western Blot Dilution: Use with Mouse TrueBlot? (ABIN1589977)
Comment

Upon initial use of this product, we recommend that the vial be inverted several times to get the beads into suspension. We recommend to use a large bore pipet to pipet up the liquid for use. For storage of the opened vial, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer.
Assay Procedure

Preparation of Immunoprecipitated Sample for SDS-PAGE:
1. Preclear cell lysate: Add 50 μL of Anti-Mouse IgG Beads and 500μl of cell lysate sample to a microcentrifuge tube and incubate on ice for
3. minutes. Spin at 10,000xg for 3 minutes and transfer the supernatant to a new microcentrifuge tube.
2. Immunoprecipitation: Add 5 μg of primary antibody to the microcentrifuge tube containing the precleared lysate. Incubate on ice for 1 hour. Add 50 μL of Anti-Mouse IgG Beads. Incubate for 1 hour on a rocking platform. Spin the microcentrifuge tube at 10000xg for 1 minute. Remove supernatant completely and wash the (pelleted) beads 3 times with 500 μL of Lysis Buffer (50mM Tris HCl pH 8.0, 150mM NaCl,1 % NP-40).
3. Prepare sample for SDS-PAGE: After the last wash, aspirate supernatant, and add 100 μL Laemmli Buffer (with 50 mM DTT or 1 %?-mercaptoethanol, final) to bead pellet. Vortex and heat to 90-100 °C for
1. minutes. Spin at 10000xg for 3 minutes, collect supernatant, and load onto the gel. Avoid loading Anti-Mouse Ig Beads.
Note: The supernatant can be stored at -20 °C for future use. After thawing, add fresh dithiothreitol and heat as above. Centrifuge the sample at 10000xg for 1 minute in a microcentrifuge tube to pellet any Anti-Mouse Ig Beads and immediately transfer an aliquot of the supernatant to gel wells.
Restrictions For Research Use only
Format Liquid
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.09 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Sensitive to light.
Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Storage 4 °C
Storage Comment Store vial at 4 °C prior to opening.
Expiry Date 6 months