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產(chǎn)品詳情

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產(chǎn)品名稱：RabbitIgGisotypecontrol(Biotin)
產(chǎn)品型號：
產(chǎn)品廠商：BioLeaf
產(chǎn)品文檔：

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簡單介紹：

RabbitIgGisotypecontrol(Biotin)

詳情介紹：

Product details
Product details
Isotype	IgG
Fragment	Fc fragment
Characteristics	Concentration Definition: by UV absorbance at 280 nm

Target details
Target details
Background	Immunoglobulin G is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains. It contains two identical class γ heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus a tetrameric quaternary structure. An antibody digested by papain yields three fragments, two Fab fragments and one Fc fragment An antibody digested by pepsin yields two fragments: a F(ab')2 fragment and a pFc' fragment. The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. This property allows antibodies to activate the immune system. Rabbit IgG F(c) is ideal for investigators involved in serum protein component research. Synonyms: Rabbit IgG Fragment, Rabbit IgG Fc, Rabbit Conjugate
Molecular Weight	150 kDa
Gene ID	100343867
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	Rabbit IgG F(c) fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Speific conditions should be optimized by user.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Restore with deionized water (or equivalent)
Concentration	1.0 mg/mL
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	Sodium azide
Precaution of Use	WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice	Aliquot to Avoid repeated freezing and thawing.
Storage	4 °C

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